The sequence of σ54 is distinctly different from that of all the σ70-like factors, some characteristics about σ54 is given below;

a) The σ54-RNA polymerase binds to the glnA promoter and immediately melt the DNA strands and initiate transcription

b) σ54-polymerase does not require ATP hydrolysis to separate the strands at a start site only require activator such as   NtrC

c) σ54-RNA polymerase initiate transcription of glnA promoter only when it is activated by NtrC

d) ATP hydrolysis is required for activation of bound σ54-RNA polymerase by phosphorylated NtrC

Which of the following from the bove combination is INCORRECT?

#Question id: 3841

#Unit 3. Fundamental Processes

The DNA replication occurs in a semi-conservative manner which means

#Question id: 3842

#Unit 3. Fundamental Processes

Which of the following statements correctly describes the difference between the leading strand and the lagging strand in DNA replication?

#Question id: 3843

#Unit 3. Fundamental Processes

In DNA replication in E. coli, the enzyme primase is used to attach a 5 to 10 base ribonucleotide strand complementary to the parental DNA strand. The RNA strand serves as a starting point for the DNA polymerase that replicates the DNA. If a mutation occurred in the primase gene, which of the following results would you expect?

#Question id: 3844

#Unit 3. Fundamental Processes

The lagging strand is characterized by a series of short segments of DNA (Okazaki fragments) that will be joined together to form a finished lagging strand. The experiments that led to the discovery of Okazaki fragments gave evidence for which of the following ideas?

#Question id: 3845

#Unit 3. Fundamental Processes

The replisome, which synthesizes DNA on both leading and lagging strands, always moves toward the replication fork. This must mean that:

#Question id: 3846

#Unit 3. Fundamental Processes

A heat-sensitive DNA ligase (25°C permissive, 37°C nonpermissive) has been used to show that DNA synthesis is discontinuous. Examination of DNA replication in the presence of [3H]-thymidine in the mutant would demonstrate which of following?