Match the Variation of Reaction Spontaneity (Sign of ΔG) given in Column I with the Signs of ΔH and ΔS given in column II.

COLUMN I	COLUMN II
	ΔH	ΔS	ΔG = ΔH – TΔS
1.	−	+	a. The reaction is both enthalpically and entropically opposed. It is nonspontaneous (endergonic) at all temperatures.
2.	−	−	b. The reaction is enthalpically opposed (endothermic) but entropically favored. It is spontaneous only at temperatures above T = ΔH/ΔS.
3.	+	+	c. The reaction is enthalpically favored but entropically opposed. It is spontaneous only at temperatures below T = ΔH/ΔS.
4.	+	−	d. The reaction is both enthalpically favored (exothermic) and entropically favored. It is spontaneous (exergonic) at all temperatures.

TLS Online TPP Program

#Question id: 23166

#Unit 13. Methods in Biology

To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.

1. FITC

2. GFP

3. Fluorescein

4. Alexa 568

Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?

TLS Online TPP Program

#Question id: 23167

#Unit 13. Methods in Biology

A secondary antibody can be generated by immunizing a goat with antibody which segment that is common to all rabbit IgG antibodies called goat anti-rabbit.

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#Question id: 23168

#Unit 13. Methods in Biology

What are the advantages of using secondary antibodies in indirect immunofluorescence?

1.  It makes easy to choose a different conjugate no matter the primary antibody

2. Enhance detection by localizing more conjugate at the antigen

3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen

Which of the following options is correct?

TLS Online TPP Program

#Question id: 23169

#Unit 13. Methods in Biology

Indirect immunofluorescence microscopy uses an unlabeled primary antibody, followed by a fluorescently labeled 

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#Question id: 23170

#Unit 13. Methods in Biology

Which epitope tags can be used to tag recombinant proteins?

1. FLAG

2. myc

3. TICK

Which of the following options is incorrect?

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#Question id: 23171

#Unit 13. Methods in Biology

Using fluorescently labeled epitope tags in microscopy actually give advantage to visualize