Bacteriophage λ, has two mode of life cycle;  lytic and lysogenic.  In the lytic growth

a) a single Cro dimer is bound to OR3; this site overlaps PRM and so Cro represses that promoter

b) Repressor bound at OR1 and OR2 blocks RNA polymerase binding at PR, repressing transcription from that promoter

c) PR and PL binds RNA polymerase and directs transcription of lytic genes

d) During lytic, PRM is on while PR (and PL) are off

Find the correct combinations of lytic growth.

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#Question id: 18852

#Unit 13. Methods in Biology

What is the starting point for selection of a suitable IEX matrix for purification of a recombinant protein?

TLS Online TPP Program

#Question id: 18853

#Unit 13. Methods in Biology

Your purification strategy of combinations of chromatography steps gives a protein preparation with a single band on SDS-PAGE. Which of the following would be best for determining the protein concentration (as mg/ml or molarity)?

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#Question id: 18854

#Unit 13. Methods in Biology

What properties of a protein does hydrophobic interaction chromatography exploit for purification?

TLS Online TPP Program

#Question id: 18855

#Unit 13. Methods in Biology

to elute target proteins from an affinity chromatography matrix, which of the following conditions would be the most appropriate?

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#Question id: 18856

#Unit 13. Methods in Biology

A scientist constructs a recombinant bacterial plasmid that contains a gene conferring resistance to the antibiotic ampicillin (amp). The scientist transforms cultures of E. coli bacteria with the recombinant plasmid, then incubates the cultures overnight on plates containing media without ampicillin (amp-) and with ampicillin (amp+).

Assuming the transformation protocol was successful, which of the following best predicts the bacterial growth pattern expected after the incubation period?

 

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#Question id: 18857

#Unit 13. Methods in Biology

Embryonic lethality that sometimes occurs in knockout models can be avoided by...