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TLS Online TPP Program

#Question id: 5001

Fossil evidence indicates that several kinds of flightless dinosaurs possessed feathers. If some of these feather-bearing dinosaurs incubated clutches of eggs in carefully constructed nests, this might be evidence supporting the claim that

#Unit 11. Evolution and Behavior

  1. dinosaurs were as fully endothermal (warm-blooded) as modern birds and mammals.

  2. their feathers originally served as insulation, and only later became flight surfaces.

  3. the earliest reptiles could fly, and the feathers of flightless dinosaurs were vestigial flight surfaces.

  4. the feathers were plucked from the bodies of other adults to provide nest-building materials.

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TLS Online TPP Program

#Question id: 23166

#Unit 13. Methods in Biology

To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.
1. FITC
2. GFP
3. Fluorescein
4. Alexa 568
Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?
TLS Online TPP Program

#Question id: 23167

#Unit 13. Methods in Biology

A secondary antibody can be generated by immunizing a goat with antibody which segment that is common to all rabbit IgG antibodies called goat anti-rabbit.
TLS Online TPP Program

#Question id: 23168

#Unit 13. Methods in Biology

What are the advantages of using secondary antibodies in indirect immunofluorescence?
1.  It makes easy to choose a different conjugate no matter the primary antibody
2. Enhance detection by localizing more conjugate at the antigen
3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen
Which of the following options is correct?
TLS Online TPP Program

#Question id: 23169

#Unit 13. Methods in Biology

Indirect immunofluorescence microscopy uses an unlabeled primary antibody, followed by a fluorescently labeled 
TLS Online TPP Program

#Question id: 23170

#Unit 13. Methods in Biology

Which epitope tags can be used to tag recombinant proteins?
1. FLAG
2. myc
3. TICK
Which of the following options is incorrect?
TLS Online TPP Program

#Question id: 23171

#Unit 13. Methods in Biology

Using fluorescently labeled epitope tags in microscopy actually give advantage to visualize