Practice Questions

TLS Online TPP Program

#Question id: 5316

Why is sequencing of eukaryotic genomes more difficult than sequencing genomes of bacteria or archaea?

#Unit 11. Evolution and Behavior

It is due to the large size of eukaryotic proteins.
Eukaryotic genomes contain sequences for hard-to-find proteins.
There is a high proportion of G-C base pairs in eukaryotic DNA, which makes sequencing difficult to complete.
The large size of eukaryotic genomes and the large amount of eukaryotic repetitive DNA make sequencing difficult.

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TLS Online TPP Program

#Question id: 18702

#Unit 13. Methods in Biology

What would be the effect on the PCR reaction if any of the following circumstances arose: 1) there are no primers in the reaction, 2) there are no dNTPs in the reaction, 3) there is no Taq polymerase in the reaction?

TLS Online TPP Program

#Question id: 18703

#Unit 13. Methods in Biology

If the weight of 1 mol bp is 660g What will the no. of moles in 4 х 10⁶ bp

TLS Online TPP Program

#Question id: 18704

#Unit 13. Methods in Biology

If the weight of 1 mol bp is 660g What will the no. of moles in human genome having 3 x 10⁹ bp

TLS Online TPP Program

#Question id: 18705

#Unit 13. Methods in Biology

What will weight in picogram in human genome

TLS Online TPP Program

#Question id: 18706

#Unit 13. Methods in Biology

Sources of thermostable DNA polymerases with proofreading (3′–5′ exonuclease) activity are given match them correctly

DNA polymerase Source

a. Tma i. Thermotoga maritima

b. Deep vent tm ii. Pyrococcus furiosus

c. Tli iii. Thermococcus litoralis

d. Pfu iv. Pyrococcus sp

e. Pwo v. Pyrococcus woesi

TLS Online TPP Program

#Question id: 18707

#Unit 13. Methods in Biology

primer dimers are formed when