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TLS Online TPP Program

#Question id: 70

The dimensions of living cells are limited, on the lower end by the minimum number of biomolecules necessary for function, and on the upper end by the rate of diffusion of solutes such as oxygen. Except for highly elongated cells, they usually have lengths and diameters in the range of

#Unit 1. Molecules and their Interaction Relevant to Biology

  1. 0.1 µm to 10 µm

  2. 0.3 µm to 30 µm

  3. 0.3 µm to 100 µm

  4. 1 µm to 300 µm

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TLS Online TPP Program

#Question id: 3813

#Unit 3. Fundamental Processes

The mechanism of DNA replication is studied in an E. coli replication fork. Which is a property of Okazaki pieces in an E. coli replication fork?
TLS Online TPP Program

#Question id: 3814

#Unit 3. Fundamental Processes

The mechanism of DNA replication is studied in an E. coli replication fork.

Which is a property of RNA primers in an E. coli replication fork?
TLS Online TPP Program

#Question id: 3815

#Unit 3. Fundamental Processes

Use the figure to answer the following question. A space probe returns with a culture of a microorganism found on a distant planet. Analysis shows that it is a carbon-based life-form that has DNA. You grow the cells in 15N medium for several generations and then transfer them to 14N medium. Which pattern in the figure would you expect if the DNA was replicated in a conservative manner?
TLS Online TPP Program

#Question id: 3816

#Unit 3. Fundamental Processes

The mechanism of DNA replication is studied in an E. coli replication fork. Which is a characteristic of an E. coli replication fork and a eukaryotic replication fork?
TLS Online TPP Program

#Question id: 3817

#Unit 3. Fundamental Processes

DNA replication is studied in a newly discovered bacterium. It takes 30 min for the bacterium to complete a round of replication at 37oC. A Meselson-Stahl-type experiment was performed. The bacteria were grown for several generations in 14N-medium and then switched to 15N-medium. The DNA molecules were then analyzed.

If the mechanism of DNA replication in this bacterium were dispersive, what results would be found when the double-stranded DNA was analyzed in a CsCl gradient after two generations in 15N-medium?
TLS Online TPP Program

#Question id: 3818

#Unit 3. Fundamental Processes

DNA replication is studied in a newly discovered bacterium. It takes 30 min for the bacterium to complete a round of replication at 37oC. A Meselson-Stahl-type experiment was performed. The bacteria were grown for several generations in 14N-medium and then switched to 15N-medium. The DNA molecules were then analyzed.

If the mechanism of DNA replication in this bacterium were semi-conservative, what results would be found when the double-stranded DNA was analyzed in a CsCl density gradient after three generations in 15N-medium?