TLS Online TPP Program

#Question id: 581


The main difference between chemical and enzyme kinetics is that

#Unit 1. Molecules and their Interaction Relevant to Biology
  1. enzyme reactions are altered by pH.

  2. enzyme reactions depend on the concentration of the substrate.

  3. enzyme reactions depend on the concentration of the enzyme and its recycling.

  4. the rate constant for the formation of products is k2.

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TLS Online TPP Program

#Question id: 19845

#Unit 13. Methods in Biology

SYBR Green qPCR method is based on____I_______ with the _____II______ relationship.

TLS Online TPP Program

#Question id: 19846

#Unit 13. Methods in Biology

Variants of PCR has been developed on the basis of___

TLS Online TPP Program

#Question id: 19865

#Unit 13. Methods in Biology

Match the following activity of variants in Column I with their variants of PCR in Column II given below;

                     COLUMN I

                        COLUMN II

 

A) Enhance efficiency

 

i) Multiplex PCR

 

B) Sequence specific amplification

 

ii) Hot-start PCR

 

C) Mutational analysis

 

iii) Megaprimer PCR

 

 

iv) qPCR with SYBR

 

 

v) AS-PCR

 

 

vi) RLM-RACE

TLS Online TPP Program

#Question id: 19866

#Unit 13. Methods in Biology

Which of the following variants of PCR has been devised to increase the specificity of PCR without lowering the efficiency by variation annealing temperature?

TLS Online TPP Program

#Question id: 19867

#Unit 13. Methods in Biology

Which of the following variants of PCR were devised to prevent non-specific amplification at low temperature?

TLS Online TPP Program

#Question id: 19868

#Unit 13. Methods in Biology

Which one of the following statements about FRET is correct?
a) FRET is possible only when the distance between donor and acceptor are within 1 to 10 A0
b) For FRET, GFP chromophore contain tripeptide sequence is -Gly-Phe-Pro-
c) qPCR based on FRET
d) Efficiency of FRET is directly proportional to the sixth power of the distance between donor and acceptor.