Which term comes next in the sequence : 2, A, 9, B, 6, C, 13, D, ? 

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#Question id: 457

#Unit 1. Molecules and their Interaction Relevant to Biology

Which are membrane associated pathways?

A) electron transport chain in eukaryotes

B) citric acid cycle in bacteria

C) photosynthesis

D) glycolysis

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#Question id: 1391

#Unit 4. Cell Communication and Cell Signaling

If an adult person has a faulty version of the human analog to ced-4 of the nematode, which of the following is most likely to result?

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#Question id: 23676

#Unit 13. Methods in Biology

What is the purpose of the heating cycle during PCR?

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#Question id: 4744

#Unit 8. Inheritance Biology

Assume that long ear lobes in humans are an autosomal dominant trait that exhibits 50% penetrance. A person who is heterozygous for long ear lobes mates with a person who is homozygous for normal ear lobes. What is the probability that their first child will have long ear lobes.

TLS Online TPP Program

#Question id: 13089

#Unit 13. Methods in Biology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 2’s plasmid is;