TLS Online TPP Program

#Question id: 598


Which might be a method used for distinguishing among several mechanistic possibilities in a multi-substrate reaction?

#Unit 1. Molecules and their Interaction Relevant to Biology
  1. Measure Km in the presence of one substrate at a time.

  2. Measure the rate with respect to one substrate while varying the concentration of another substrate.

  3. Measure the rate while adding inactivators for all but one substrate.

  4. Any of the above

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TLS Online TPP Program

#Question id: 4340

#Unit 3. Fundamental Processes

There is a mutation that is found in E. coli in the repressor that results in a molecule known as a super-repressor because it represses the lac operon permanently. Which of the following characteristics would you expect to observe in such a mutant?

TLS Online TPP Program

#Question id: 4341

#Unit 3. Fundamental Processes

Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome. If a researcher moves the promoter for the lac operon to the region between the beta galactosidase (lacZ) gene and the permease (lacY) gene, which of the following results would be most likely?

TLS Online TPP Program

#Question id: 4342

#Unit 3. Fundamental Processes

Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome. If a researcher moves the operator to the far end of the operon, past the transacetylase (lacA) gene, which of the following processes would likely occur when the cell is exposed to lactose?

TLS Online TPP Program

#Question id: 4343

#Unit 3. Fundamental Processes

Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome. If a researcher moves the repressor gene (lacI), along with its promoter, to a position at some several thousand base pairs away from its normal position, which of the following results would be expected?

TLS Online TPP Program

#Question id: 4344

#Unit 3. Fundamental Processes

How does the transcription of structural genes in an inducible operon occur?

TLS Online TPP Program

#Question id: 4345

#Unit 3. Fundamental Processes

For a repressible operon to be transcribed, which of the following conditions must occur?