Oxidative decarboxylation of pyruvate to acetyl-CoA by the PDH complex, how the pyruvate dehydrogenase complex carries out the five consecutive reactions in the decarboxylation and dehydrogenation of pyruvate?

 A) Pyruvate reacts with the bound thiamine pyrophosphate (TPP) of pyruvate dehydrogenase, undergoing decarboxylation to the hydroxyethyl derivative

 B) A transesterification in which the —SH group of CoA replaces the —SH group of E2 to yield acetyl-CoA and the fully reduced (dithiol) form of the lipoyl group

 C) The reduced FADH2 of E3 transfers a hydride ion to NAD1 , forming NADH, the enzyme complex is now ready for another catalytic cycle

 D) The transfer of two electrons and the acetyl group from TPP to the oxidized form of the lipoyllysyl group of the core enzyme, dihydrolipoyl transacetylase, to form the acetyl thioester of the reduced lipoyl group

 E) Dihydrolipoyl dehydrogenase promotes transfer of two hydrogen atoms from the reduced lipoyl groups of E2 to the FAD prosthetic group of E3 , restoring the oxidized form of the lipoyllysyl group of E2

 Find out the correct sequence of action of this complex enzyme?

#Question id: 5248

#Unit 8. Inheritance Biology

Which of the following is a description of generalized transduction?

#Question id: 5249

#Unit 8. Inheritance Biology

What is the best explanation for why Bernard Davis didn't observe generalized transduction in his U tube experiments?

#Question id: 5250

#Unit 8. Inheritance Biology

A researcher would like to map the location of galE and trpA genes in a new species of bacterium that appears to be closely related to E. coli. He decides to use cotransduction, and generatesnappropriate donor and recipient strains. He is disappointed when cotransduction is not seen in his experiement. What is the most reasonable explanation for this situation?

#Question id: 5251

#Unit 8. Inheritance Biology

What do transduction, transformation, and conjugation have in common?

#Question id: 5252

#Unit 8. Inheritance Biology

Which type(s) of genetic transfer lead to incorporation of new DNA into the bacterial chromosome?

I. Conjugation mediated by F factor

II. Hfr mediated conjugation

III. Transduction

IV. Transformation

#Question id: 5253

#Unit 8. Inheritance Biology

In a hypothetical situation, the genes for sex pilus construction and for tetracycline resistance are located together on the same plasmid within a particular bacterium. If this bacterium readily performs conjugation involving a copy of this plasmid, then the resultshould be