Methotrexate is an inhibitor of dihydrofolate reductase. Administration of methotrexate would inhibit:

1. de novo synthesis of UMP.

2. conversion of dUMP to dTMP.

3. conversion of IMP to GMP.

4. de novo synthesis of IMP

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#Question id: 13059

#Unit 13. Methods in Biology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

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#Question id: 4951

#Unit 11. Evolution and Behavior

The synthesis of new DNA requires the prior existence of oligonucleotides to serve as primers. On Earth, these primers are small RNA molecules. This latter observation is evidence in support of the hypothesized existence of

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#Question id: 24543

#Unit 9. Diversity of Life Forms

During an experiment observed, a plant species that descended from a white-flower-bearing ancestor eventually became a red-flower-bearing species and made that species “unique”. The red-flower trait is an

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#Question id: 4100

#Unit 3. Fundamental Processes

some pre-mRNAs are spliced by an alternative, low-abundance form of the spliceosome. This rare form contains some components common to the major spliceosome, but it contains other unique components such as

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#Question id: 14509

#Unit 13. Methods in Biology

Aishwarya studies either computer science or mathematics everyday. If she studies computer science on a day, then the probability that she studies mathematics the next day is 0.6. If she studies mathematics on a day, then the probability that she studies computer science the next day is 0.4. Given that Aishwarya studies computer science on Monday, what is the probability that she studies computer science on Wednesday ?