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#Unit 2. Cellular Organization
NtrC controls expression of genes involved in nitrogen metabolism by inducing a conformational change in a pre-bound RNA polymerase, triggering transition to the open complex.
I. Under low nitrogen level condition, NtrC is phosphorylated by a kinase, NtrB,
II. NtrC (bound to its sites upstream) interacts directly with s54. This requires that the DNA between the activator binding sites and the promoter form a loop to accommodate the interaction
III. Active NtrC binds four sites located approximately 150 bp upstream of the glnA gene.
IV. NtrC itself has an ATPase activity which provides the energy needed to induce a conformational change in polymerase.
Correct series of Activation by NtrC is;