TLS Online TPP Program

#Question id: 32367


Which of the following is a common technique used to study actin assembly in vitro?

#Unit 2. Cellular Organization
  1. Fluorescence microscopy
  2. Pyrene-actin polymerization assay
  3. Western blotting
  4. Electrophysiology
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TLS Online TPP Program

#Question id: 34090

#Unit 3. Fundamental Processes

A. DnaA is in the active form only when bound to ATP.
B. All three13-bp repeats of origin must be opened for the initiation
C. 9-bp repeats are found outside of 245 bp origin 
D. DnaA has a weak intrinsic activity that converts the ATP to ADP, this is enhanced by a factor termed Had

Choose true and false

TLS Online TPP Program

#Question id: 34091

#Unit 3. Fundamental Processes

DnaA has intrinsic ATPase activity that hydrolyzes ATP to ADP and thus inactivates itself when the initiation stage ends. This ATPase activity is stimulated by 
A. Membrane phospholipids which helps in ATP hydrolysis
B. dsDNA which is substrate for DnaA
C. Membrane phospholipids which provide ATP in exchange of ADP
D. ssDNA which is created in first step

Which of the following is correct combination

TLS Online TPP Program

#Question id: 34092

#Unit 3. Fundamental Processes

A. SeqA binds to hemimethylated DNA and is required for delaying rereplication.
B. As the origins are hemimethylated they bind to the cell membrane and may be unavailable to methylases.
C. The dat locus contains DnaA-binding sites that titrate availability of DnaA protein.
D. Hda protein is recruited to the replication origin to convert DnaA-ATP to DnaA-ADP.

Choose true and false

TLS Online TPP Program

#Question id: 34093

#Unit 3. Fundamental Processes

Quite large regions of the eukaryotic chromosome can be characterized as “early replicating” or “late replicating,”. These site can be recognised by

TLS Online TPP Program

#Question id: 34094

#Unit 3. Fundamental Processes

The rate of eukaryotic replication is 2000 bp/min and replicon size is 40kb. How much time it will take replicate entire human chromosome

TLS Online TPP Program

#Question id: 34095

#Unit 3. Fundamental Processes

The origin recognition complex (ORC) is a highly conserved complex found in all eukaryotes. It is composed of six proteins and it binds to