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TLS Online TPP Program

#Question id: 7155

What does it tell us about aging that cells from different organisms are capable of a fixed number of divisions in culture, and that number of divisions reflects the relative life-span of the organism?

#Unit 5. Developmental Biology
  1. The cells from these different organisms are not all equally adapted to growth in culture.
  2. In each organism, the cells seem to be capable of a genetically determined number of divisions, after which the organism undergoes senescence and dies.
  3. Cells growing in culture live for the same number of years that the organism from which they are derived would be expected to live, indicating a genetically determined life span.
  4. The conditions cells encounter in culture lead to oxidative damage which reflects the kind of damage that normally leads to aging in the organism.
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TLS Online TPP Program

#Question id: 23148

#Unit 13. Methods in Biology

There are two proteins A and B to be visualized under fluorescence microscopy. Both of them are excited with same wavelength of light but A emits longer wavelength. Hence we can conclude that 
1. A is denser molecule than B
2. B is denser molecule than A
3. A is lighter molecule than B
4. B is lighter molecule than A
Which of the following option is incorrect?
TLS Online TPP Program

#Question id: 23149

#Unit 13. Methods in Biology

In fluorescence microscopy a usually used fluorochrome that is fura-2 is sensitive to which ions?
TLS Online TPP Program

#Question id: 23150

#Unit 13. Methods in Biology

How fura-2 dye moves inside the cell through plasma membrane?
TLS Online TPP Program

#Question id: 23151

#Unit 13. Methods in Biology

If the concentration of Ca2+ is high inside the cell and fura-2 dye is used to map the concentration which of the following conditions will prevail?
1. Fluorescence will decrease
2.  Fluorescence will increase
3. Emission of light at higher wavelength
4. Emission of light at lower wavelength
Which of the following options are correct?
TLS Online TPP Program

#Question id: 23152

#Unit 13. Methods in Biology

A fluorescent dye SNARF-1 that is sensitive to H+ concentrations can be used to monitor the cytosolic pH of 
1. Alkaline cells
2. Acidic cells 
3. Dead cells
4. Live cells
Which of the following options are incorrect?
TLS Online TPP Program

#Question id: 23153

#Unit 13. Methods in Biology

SNARF-1is a fluorescent dye used to measure which ion concentration?