TLS Online TPP Program

#Question id: 7231


Kragl and colleagues (2009) performed an experiment in which they transplanted limb tissue from a salamander whose cells expressed green fluorescent protein (GFP) into different regions of limbs of normal salamanders that did not have the GFP transgene. If they transplanted the GFP-expressing limb cartilage into a salamander limb that did not contain the GFP transgene, the GFP-expressing cartilage would integrate normally into the limb skeleton. They later amputated the limb through the region containing GFP-marked cartilage cells. The blastema was found to contain GFP-expressing cells, and when the blastema differentiated, the only GFP-expressing cells found were in the limb cartilage. Similarly, GFP-marked muscle cells gave rise only to muscle, and GFP-marked epidermal cells only produced the epidermis of the regenerated limb. Which of the following phenomenon is proved according to experiment?

#Unit 5. Developmental Biology
  1. These results suggest that nAG alone is the primary mitogen responsible for nerve-dependent regeneration.
  2. The blastema is not a collection of unspecified multipotent progenitor cells. Rather, the cells retain their specification, and the blastema is a heterogeneous assortment of restricted progenitor cells.
  3. The blastema is a collection of unspecified multipotent progenitor cells. Rather, the cells lost their specification, and the blastema is a homogeneous assortment of restricted progenitor cells.
  4. Salamanders accomplish epimorphic regeneration by cell dedifferentiation to form a regeneration blastema, which in this case is an aggregation of relatively undifferentiated cells derived from the originally differentiated tissue that then proliferates and redifferentiates into the new limb parts.

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TLS Online TPP Program

#Question id: 3977

#Unit 3. Fundamental Processes

Which one of the following statements about eukaryotic RNA polymerases is correct?

TLS Online TPP Program

#Question id: 3978

#Unit 3. Fundamental Processes

The 5'-terminal cap structure of eukaryotic mRNAs is a(n):

TLS Online TPP Program

#Question id: 3979

#Unit 3. Fundamental Processes

“Housekeeping genes” in bacteria are commonly expressed constitutively, but not all of these genes are expressed at the same level (the same number of molecules per cell).  The primary mechanism responsible for variations in the level of constitutive enzymes from different genes is that:

TLS Online TPP Program

#Question id: 3980

#Unit 3. Fundamental Processes

Which of the following statements correctly describes promoters in E. coli?

TLS Online TPP Program

#Question id: 3981

#Unit 3. Fundamental Processes

The diagram below represents a hypothetical operon in the bacterium E. coli.  The operon consists of two structural genes (A and B), which code for the enzymes A-ase and B-ase, respectively, and also includes P (promoter) and O (operator) regions as shown.

 When a certain compound (X) is added to the growth medium of E. coli, the separate enzymes A-ase and B-ase are both synthesized at a 50-fold higher rate than in the absence of X.  (X has a molecular weight of about 200.)  Which of the following statements is true of the operon decribed above?

TLS Online TPP Program

#Question id: 3982

#Unit 3. Fundamental Processes

The diagram below represents a hypothetical operon in the bacterium E. coli.  The operon consists of two structural genes (A and B) that code for the enzymes A-ase and B-ase, respectively, and also includes P (promoter) and O (operator) regions as shown.

 When a certain compound (X) is added to the growth medium of E. coli, the separate enzymes A-ase and B-ase are both synthesized at a 50-fold higher rate than in the absence of X (which has a molecular weight of about 200).  Which one of the following statements is true of such an operon?