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TLS Online TPP Program

#Question id: 9229

Which of the following is a generally accurate statement about the current research regarding forest fragmentation?

#Unit 9. Diversity of Life Forms

  1. Fragmented forests promote biodiversity because they result in the combination of forestedge species and forest-interior species.

  2. In fragmented forests, the number of forested-adapted species tend to decline and the number of edge species tend to increase.

  3. Fragmented forests are the goal of conservation biologists who design wildlife reserves.

  4. The disturbance of timber extraction causes the species diversity to increase because of the new habitats created.

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TLS Online TPP Program

#Question id: 13050

#Unit 13. Methods in Biology

Which of the following expressions can be used in insects?
TLS Online TPP Program

#Question id: 13051

#Unit 13. Methods in Biology

Initiation of translation in higher eukaryotic organisms depends on a specific sequence of nucleotides surrounding the start (AUG) codon in the mRNA called……..
TLS Online TPP Program

#Question id: 13052

#Unit 13. Methods in Biology

What would be the effect on the PCR reaction if any of the following circumstances arose: 1) there are no primers in the reaction, 2) there are no dNTPs in the reaction, 3) there is no Taq polymerase in the reaction?
TLS Online TPP Program

#Question id: 13053

#Unit 13. Methods in Biology

What would the generally expected effect on the PCR reaction be of adjustments that increase the temperature of the annealing phase and the length of the elongation phase?
TLS Online TPP Program

#Question id: 13054

#Unit 13. Methods in Biology

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?
TLS Online TPP Program

#Question id: 13055

#Unit 13. Methods in Biology

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following will provide least specific amplification in qPCR?