TLS Online TPP Program

#Question id: 21413


Aqueous two phase partitioning (ATPS) is used for the recovery of an enzyme from the cell free culture filtrate on addition of PEG2000 and dextran. The mixture separates into two phases with a partition coefficient for the enzyme = 4.2. The maximum possible enzyme recovery, when the volume ratio of the upper to lower phases is 5.0 will be:

#Unit 12. Biochemical Engineering
  1. 68%
  2. 76%
  3. 85%
  4. 90%