Practice Questions

TLS Online TPP Program

#Question id: 10146

Light-dependent ion movements modulate enzymes of the Calvin–Benson cycle such as : rubisco, fructose 1,6-bisphosphatase, sedoheptulose 1,7-bisphosphatase, and phosphoribulokinase what will happen Upon illumination;

#Unit 6. System Physiology – Plant

flow of protons from the thylakoid lumen into the stroma is coupled to release of Mg2+ from the intrathylakoid space to the stroma
light actuated ion fluxes decrease the stromal concentration of protons (the pH increases from 7 to 8) and decrease that of Mg2+ by 2 to 5 mM
The light-mediated decrease of pH and the concentration of Mg2+ activate enzymes of the Calvin–Benson cycle that require Mg2+ for catalysis and are more active at pH 7 than at pH 8
The modifications of ionic composition of the chloroplast stroma are reversed rapidly upon darkening

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TLS Online TPP Program

#Question id: 23159

#Unit 13. Methods in Biology

Rhodamine is a fluorescent dye which emits a colour?

TLS Online TPP Program

#Question id: 23160

#Unit 13. Methods in Biology

Texas is a fluorescent dye which emits a colour?

TLS Online TPP Program

#Question id: 23161

#Unit 13. Methods in Biology

A classically used fluorescent dye which emits a orange light is?

TLS Online TPP Program

#Question id: 23162

#Unit 13. Methods in Biology

Match the fluorescent dyes in column I with emission light in column II

Column I Column II

1. Texas a. far red

2. Fluorescein b. red

3. Rhodamine c. blue

4. Cy5 d. green

Which of the following option comprises all correct matches?

TLS Online TPP Program

#Question id: 23163

#Unit 13. Methods in Biology

Out of the given below alexa dyes which one will emit in red light range?

TLS Online TPP Program

#Question id: 23164

#Unit 13. Methods in Biology

Out of the given below alexa dyes which one will emit in Blue light range?