Rubisco catalyzes both the carboxylation and the oxygenation of ribulose 1,5-bisphosphate, the oxygenase activity of rubisco causes partial loss of the carbon fixed by the Calvin–Benson cycle, to avoid loss a reaction takes place such as photorespiration, The following statements about photorespiration.

a.) To avoid drain of carbon out of the Calvin–Benson cycle is metabolized through the C2 oxidative photosynthetic carbon cycle

b.) In photorespiration involves chloroplasts, leaf peroxisomes, and mitochondria

c.) During oxygenation process Rubisco fixes oxygen at high temperature

d.) One of the rection of the C2 cycle is Glycine converted into the serine is takes place in peroxisome

Which of following from above combination is CORRECT?

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#Question id: 23164

#Unit 13. Methods in Biology

Out of the given below alexa dyes which one will emit in Blue light range?

TLS Online TPP Program

#Question id: 23165

#Unit 13. Methods in Biology

For visualizing two proteins which are localized in nucleus through fluorescence microscopy which two sets of dyes can be best used for clear visual?

TLS Online TPP Program

#Question id: 23166

#Unit 13. Methods in Biology

To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.

1. FITC

2. GFP

3. Fluorescein

4. Alexa 568

Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?

TLS Online TPP Program

#Question id: 23167

#Unit 13. Methods in Biology

A secondary antibody can be generated by immunizing a goat with antibody which segment that is common to all rabbit IgG antibodies called goat anti-rabbit.

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#Question id: 23168

#Unit 13. Methods in Biology

What are the advantages of using secondary antibodies in indirect immunofluorescence?

1.  It makes easy to choose a different conjugate no matter the primary antibody

2. Enhance detection by localizing more conjugate at the antigen

3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen

Which of the following options is correct?

TLS Online TPP Program

#Question id: 23169

#Unit 13. Methods in Biology

Indirect immunofluorescence microscopy uses an unlabeled primary antibody, followed by a fluorescently labeled