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TLS Online TPP Program
#Question id: 7504
#General Aptitude
Two train 100 metres and 120 metres long are running in the same direction with speed of 72 km/hr and 54 km/hr. In how much time will the first train cross the second?
TLS Online TPP Program
#Question id: 27083
#Unit 1. Molecules and their Interaction Relevant to Biology
Which of the following is correctly describing the differences between β Turn type I & β Turn type II
(1) type I & II i + 1 values differ only in psi values
(2) type I & II i + 1 values differ only in phi values
(3) type I & II i + 2 values differ only in phi values
(4) type I & II i + 2 values differ only in psi values
TLS Online TPP Program
#Question id: 27036
#Unit 10. Ecological Principles
Mafia hypothesis based on
a) Taking food by theft
b) destroying all eggs of their host’s nests
c) Changing color of host making more susceptible to being eaten
TLS Online TPP Program
#Question id: 11888
#General Aptitude
If the ratio of two numbers is 2 : 3 and their LCM is 54, then the sum of the two numbers is
TLS Online TPP Program
#Question id: 13057
#Unit 13. Methods in Biology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
How primer concentration leads to decrease in PCR efficiency?