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TLS Online TPP Program

#Question id: 10285

Some of the statements given shows the activity of PFK-1 and PFK-2, which of the following statements incorrectly shown these enzyme activity?

#Unit 6. System Physiology – Plant
  1. Fructose 2,6-bisphosphate is formed by phosphorylation of fructose 6-phosphate, catalyzed by phosphofructokinase-2 (PFK-2), and is broken down by fructose 2,6-bisphosphatase (FBPase-2)
  2. fructose 2,6-bisphosphate is an allosteric activator of PFK-1 activity
  3. PFK-1 is virtually inactive in the absence of fructose2,6-bisphosphate, fructose 2,6-bisphosphate has the opposite effect on FBPase-1: it reduces its affinity for its substrate, thereby slowing gluconeogenesis

  4. cAMP-dependent protein kinase, which transfers a phosphoryl group from ATP to the bifunctional protein PFK-1/FBPase-1. Phosphorylation of this protein enhances its FBPase-1 activity and inhibits its PFK-1 activity

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TLS Online TPP Program

#Question id: 32313

#Unit 2. Cellular Organization

Why does actin subunit addition outpace nucleotide hydrolysis at the plus end but not at the minus end?
TLS Online TPP Program

#Question id: 1445

#Unit 4. Cell Communication and Cell Signaling

Germ-line mutations in which of the following has(have) been implicated in hereditary cancers?
TLS Online TPP Program

#Question id: 1337

#Unit 4. Cell Communication and Cell Signaling

The promotion of metastasis by the loss of E-cadherin suggests that E-cadherin inhibits metastasis. Which one of the following types of proteins are most likely involved in the inhibition of metastasis by E-cadherin?
TLS Online TPP Program

#Question id: 13062

#Unit 13. Methods in Biology

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?
TLS Online TPP Program

#Question id: 15671

#Unit 2. Cellular Organization

The lag phase of the bacterial growth curve is marked by