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#Question id: 10334


N2 combines with hydrogen to form ammonia under elevated temperature (about 200°C) and high pressure (about 200 atmospheres) and in the presence of a metal catalyst (usually iron). The extreme conditions are required to overcome the high activation energy of the reaction. This nitrogen fixation reaction, called

#Unit 6. System Physiology – Plant
  1. Aldol condensation  

  2. Haber–Bosch process
  3. claisen condensation
  4. Asadaheliwell pathway
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#Question id: 19884

#Unit 13. Methods in Biology

The first successful transformation of rDNA molecules into a bacterium was carried out by

TLS Online TPP Program

#Question id: 19885

#Unit 13. Methods in Biology

The plasmid used by Cohen and Boyer fr their transformation experiment was

TLS Online TPP Program

#Question id: 19886

#Unit 13. Methods in Biology

Gene cloning refers to the
I) Production of large number of copies of the gene being cloned
II) Production of asexual progeny from a single individual or a cell 
III) Mechanism of intake of DNA fragments from the surrounding medium by a cell

TLS Online TPP Program

#Question id: 19887

#Unit 13. Methods in Biology

Paul Berg’s gene splicing experiment created the first rDNA molecules which was a

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#Question id: 19888

#Unit 13. Methods in Biology

There are some typical enzyme used in the manipulation of nucleic acid;

                 

                 ENZYMES

 

USE IN NUCLEIC ACID MANIPULATION

 

A) klenow

 

i) DNA-dependent RNA polymerase

 

B) T4 DNA polymerase

 

ii) Single-strand specific nuclease

 

C) Taq DNA polymerase

 

iii) DNA pol I lacks 5’→ 3’ exonuclease activity

 

D) T7 RNA polymerase

 

iv) Thermostable DNA polymerase used in PCR

 

E) S1 nuclease

 

v) Lack  5’→ 3’ exonuclease activity

TLS Online TPP Program

#Question id: 19889

#Unit 12. Applied Biology

The genetic monitoring and disease diagnosis are examples for ______________________ sensor?