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#Question id: 733

Which one of the statements on protein conformation, detailed below is INCORRECT?

#Unit 1. Molecules and their Interaction Relevant to Biology

  1. All types of B turns exist with both positive and negative dihydral angle values.

  2. PPII or PGII helix are found in proteins but they are not considered as equivalent secondary structure elements.

  3. Dihydral angles of extended peptide chain exist near 0o point in Ramachandran plot.

  4. PPII helices are stabilized by main chain-water hydrogen bonds

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TLS Online TPP Program

#Question id: 18958

#Unit 13. Methods in Biology

The number of clones to represent this fragment in genomic library with a probability of 95% are
TLS Online TPP Program

#Question id: 18959

#Unit 13. Methods in Biology

 Vectors used for construction of genomic libraries, and their capacity is given match them correctly
A- λ EMBL series                                                                                   I - 300kb - 1,400 kb
B - Cosmids                                                                                                 II - 45 kb
C – BACs and YACs (high capacity vectors)                                             III - 20kb
TLS Online TPP Program

#Question id: 18960

#Unit 13. Methods in Biology

cDNA library do not require very large vectors hence which of the following is commonly used in cDNA library
TLS Online TPP Program

#Question id: 18961

#Unit 13. Methods in Biology

When a cDNA library is generated by first removing those mRNA or cDNA sequences that are common to two sources, e.g., two different Cell types, such a library is called 
TLS Online TPP Program

#Question id: 18962

#Unit 13. Methods in Biology

1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA,

2. Their large size makes the handling procedures during cloning tedious and cumbersome

3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned.

These three problems have been resolved by deleting 
TLS Online TPP Program

#Question id: 18963

#Unit 13. Methods in Biology

The use of wild type pTi as a vector presents the following three problems.
1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA, which causes a disorganised growth and a loss of regeneration potentiaLof the cells having T-DNA in their genomes.
2. Their large size makes the handling procedures during cloning tedious and cumbersome.
3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned
In the above mention’s problems, 1 and 3 can removed by disarming and creating MCS, to overcome from 2nd problem need to develop