Mechanical barriers provide a first line of defence against insect pests and pathogens, these mechanical barriers developed by plants;

COLUMN A	COLUMN B
A. Thorns	i) It can be found on the stem and petioles of roses and are formed by the epidermis
B. Spines	ii) Found on a lemon tree (Citrus sp.) are modified branches, as can be seen by their position in the axil of a leaf
C. Prickles	iii) Found on shoot and leaves of tomato (Solanum lycopersicum) are derived from epidermal cells
D. Trichomes	iv) which are characteristic of cacti (Opuntia spp.) in the New World, are modified leaves

Match correctly following combination of barrier developed by plants ;

TLS Online TPP Program

#Question id: 23150

#Unit 13. Methods in Biology

How fura-2 dye moves inside the cell through plasma membrane?

TLS Online TPP Program

#Question id: 23151

#Unit 13. Methods in Biology

If the concentration of Ca2+ is high inside the cell and fura-2 dye is used to map the concentration which of the following conditions will prevail?

1. Fluorescence will decrease

2.  Fluorescence will increase

3. Emission of light at higher wavelength

4. Emission of light at lower wavelength

Which of the following options are correct?

TLS Online TPP Program

#Question id: 23152

#Unit 13. Methods in Biology

A fluorescent dye SNARF-1 that is sensitive to H+ concentrations can be used to monitor the cytosolic pH of 

1. Alkaline cells

2. Acidic cells 

3. Dead cells

4. Live cells

Which of the following options are incorrect?

TLS Online TPP Program

#Question id: 23153

#Unit 13. Methods in Biology

SNARF-1is a fluorescent dye used to measure which ion concentration?

TLS Online TPP Program

#Question id: 23154

#Unit 13. Methods in Biology

When the organelles pH is acidic the how the SNARF-1 dye measures this acidity?

TLS Online TPP Program

#Question id: 23155

#Unit 13. Methods in Biology

Match the following ions in column I with their relevant dyes column II.

Column I                                                       Column II

1. K+                                                                 a. Fura-2

2. Ca2+                                                             b. SNARF-1

3. H+                                                                 c. Ion potassium green

Which of the following options is correct?