TLS Online TPP Program

#Question id: 796


Which statement best describes the distinction between the purposes of the two RNAi pathways within the eukaryotic cell?

#Unit 1. Molecules and their Interaction Relevant to Biology
  1. miRNA-mediated gene silencing represents a back-up pathway should siRNA-mediated gene silencing be unsuccessful at silencing the target dsRNA

  2. siRNA and miRNA-mediated gene silencing pathways must both be active to successfully silence the target dsRNA

  3. miRNA and siRNA-mediated gene silencing represent evolutionary-independent pathways which confer identical silencing mechanisms on the target dsRNA

  4. siRNA-mediated gene silencing represents a cell defence mechanism against exogenous dsRNA; miRNA-mediated gene silencing is an integral gene expression regulation process

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TLS Online TPP Program

#Question id: 4552

#Unit 3. Fundamental Processes

The sequence of σ54 is distinctly different from that of all the σ70-like factors, some characteristics about σ54 is given below;

a) The σ54-RNA polymerase binds to the glnA promoter and immediately melt the DNA strands and initiate transcription

b) σ54-polymerase does not require ATP hydrolysis to separate the strands at a start site only require activator such as   NtrC

c) σ54-RNA polymerase initiate transcription of glnA promoter only when it is activated by NtrC

d) ATP hydrolysis is required for activation of bound σ54-RNA polymerase by phosphorylated NtrC

Which of the following from the bove combination is INCORRECT?

TLS Online TPP Program

#Question id: 4553

#Unit 3. Fundamental Processes

The NtrC protein (nitrogen regulatory protein C)—stimulates transcription of the glnA gene, this protein is an activator of σ54-RNA polymerase. Which of the following given characteristics about this protein is incorrect?

TLS Online TPP Program

#Question id: 4554

#Unit 3. Fundamental Processes

In which studies have shown that phosphorylated NtrC bound at enhancers and σ54-polymerase bound at the promoter interact directly, forming a loop in the DNA between the binding sites?

TLS Online TPP Program

#Question id: 4555

#Unit 3. Fundamental Processes

The σ54-RNA polymerase binds to the glnA promoter but does not melt the DNA strands and initiate transcription until it is activated by

TLS Online TPP Program

#Question id: 4557

#Unit 3. Fundamental Processes

In eukaryotic transcription RNA polymerase II is transcribed

TLS Online TPP Program

#Question id: 4558

#Unit 3. Fundamental Processes

Classes of RNA transcribed by the three eukaryotic nuclear RNA Polymerase;

                    Polymerase

             RNA Transcribed     

A. RNA polymerase I                   

i) tRNAs, 5S rRNA, snRNA, U6, 7S RNA

B. RNA polymerase II

ii) Pre-rRNA 28S, 18SrRNA,  5.8SrRNAs

C. RNA polymerase III

iii) mRNA, snRNAs, siRNAs, miRNAs

 Match the following correct combination given above combinations;