The rate of O2 uptake by the breathing organ depends on the

TLS Online TPP Program

#Question id: 18958

#Unit 13. Methods in Biology

The number of clones to represent this fragment in genomic library with a probability of 95% are

TLS Online TPP Program

#Question id: 18959

#Unit 13. Methods in Biology

 Vectors used for construction of genomic libraries, and their capacity is given match them correctly

A- λ EMBL series	I - 300kb - 1,400 kb
B - Cosmids	II - 45 kb
C - BACs and YACs (high capacity vectors)	III - 20kb

TLS Online TPP Program

#Question id: 18960

#Unit 13. Methods in Biology

cDNA library do not require very large vectors hence which of the following is commonly used in cDNA library

TLS Online TPP Program

#Question id: 18961

#Unit 13. Methods in Biology

When a cDNA library is generated by first removing those mRNA or cDNA sequences that are common to two sources, e.g., two different Cell types, such a library is called 

TLS Online TPP Program

#Question id: 18962

#Unit 13. Methods in Biology

1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA,

2. Their large size makes the handling procedures during cloning tedious and cumbersome

3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned.

These three problems have been resolved by deleting 

TLS Online TPP Program

#Question id: 18963

#Unit 13. Methods in Biology

The use of wild type pTi as a vector presents the following three problems.

1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA, which causes a disorganised growth and a loss of regeneration potentiaLof the cells having T-DNA in their genomes.

2. Their large size makes the handling procedures during cloning tedious and cumbersome.

3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned

In the above mention’s problems, 1 and 3 can removed by disarming and creating MCS, to overcome from 2nd problem need to develop