#Question id: 2838
#Unit 2. Cellular Organization
You want to study the potential interaction between nucleosome-bound DNA and a specific histone deacetylase. You decide to perform an electrophoretic mobility shift assay (EMSA). You use a 32P end-labelled, linear template DNA that contains two nucleosome positioning sites. You assemble two nucleosomes on the DNA template before incubation with and without the histone deacetylase. For some reactions, you use unmodified nucleosomes. For other reactions, you use nucleosomes that are methylated at lysine 36 of the histone protein H3.
A. The histone deacetylase binds nucleosome bound-DNA in lanes 1, 2, 3, and 4.
B. The histone deacetylase binds nucleosome bound-DNA in lanes 3& 4.
C. The histone deacetylase seems to recognize methylated nucleosomes at lysine 36 of histone H3 in lane 1, 2 & 3 better than unmethylated nucleosomes in lane 4 &5
D. The histone deacetylase seems to recognize methylated nucleosomes at lysine 36 of histone H3 in lane 1 & 2 better than unmethylated nucleosomes in lane 3 &4
#Question id: 29471
#Unit 6. System Physiology – Plant
Strigolactone promote symbiotic interactions with arbuscular mycorrhizal fungi, facilitating the uptake of ion from soil is
#Question id: 2869
#Unit 2. Cellular Organization
Galactose imported into the yeast cell is converted to galactose 6-phosphate by a pathway involving six enzymes, whose genes are scattered over three chromosomes. Transcription of these genes is regulated by the combined actions of the proteins Gal4p, Gal80p, and Gal3p,
i. GAL3 |
a. Interaction with the Gal80p-Gal4p complex and activates Gal4p. |
ii. GAL4 |
b. Recruits SAGA, Mediator, and TFIID to the galactose promoters, leading to DNA polymerase II recruitment and initiation of transcription |
iii. GAL80 |
c. Transcriptional inhibitor |
d. Transcriptional activator |
#Question id: 598
#Unit 1. Molecules and their Interaction Relevant to Biology
Which might be a method used for distinguishing among several mechanistic possibilities in a multi-substrate reaction?
#Question id: 26815
#Unit 8. Inheritance Biology