A 65-year-old man has a heart attack and experiences cardiopulmonary arrest while being transported to the emergency room. The following laboratory values are obtained from arterial blood:

plasma pH = 7.12,

plasma PCO2 = 60mm Hg, and

plasma HCO3− concentration = 19 mEq/L.

Which of the following best describes his acid-base disorder?

TLS Online TPP Program

#Question id: 13050

#Unit 13. Methods in Biology

Which of the following expressions can be used in insects?

TLS Online TPP Program

#Question id: 13051

#Unit 13. Methods in Biology

Initiation of translation in higher eukaryotic organisms depends on a specific sequence of nucleotides surrounding the start (AUG) codon in the mRNA called……..

TLS Online TPP Program

#Question id: 13052

#Unit 13. Methods in Biology

What would be the effect on the PCR reaction if any of the following circumstances arose: 1) there are no primers in the reaction, 2) there are no dNTPs in the reaction, 3) there is no Taq polymerase in the reaction?

TLS Online TPP Program

#Question id: 13053

#Unit 13. Methods in Biology

What would the generally expected effect on the PCR reaction be of adjustments that increase the temperature of the annealing phase and the length of the elongation phase?

TLS Online TPP Program

#Question id: 13054

#Unit 13. Methods in Biology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

TLS Online TPP Program

#Question id: 13055

#Unit 13. Methods in Biology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?