TLS Online TPP Program

#Question id: 878


Can a peptide bind more strongly to an unfolded than the same protein in a folded state?

#Unit 1. Molecules and their Interaction Relevant to Biology
  1. It will depend on the balance of favourable interactions (enthalpy) versus loss in conformational entropy. For the folded protein, the loss in conformational entropy is less upon binding.

  2. If the increased interactions for the unfolded protein can less than compensate the increased loss in conformational entropy then the net Gibbs free energy will be favourable.

  3. The unfolding do not cause the the appearance of hydrophobic sites of the surface of protein, so if the interaction of the peptide with the protein is depending on the hydrophobic site.

  4. If the binding site changed due to unfolding the binding will be stronger.

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TLS Online TPP Program

#Question id: 11720

#Unit 6. System Physiology – Plant

The embryo, which will grow into the new seedling, has a specialized absorptive organ that is___

TLS Online TPP Program

#Question id: 11721

#Unit 6. System Physiology – Plant

The induction of α-amylase synthesis in barley aleurone layers by gibberellin, given some statements about after the induction of GA into the aleurone layer, and allows some transcriptional gene function inside the nucleus such as;

a) GA-MYB is a negative regulator of α-amylase transcription

b) The sequence of the gibberellic acid response element (GARE) in the α-amylase gene promoter is similar to the DNA sequence to which MYB proteins bind.

c) Synthesis of GA-MYB mRNA begins to increase as early as 1 h after gibberellin treatment, preceding the increase in a-amylase mRNA by several  hours

d) A mutation in the GARE that prevents MYB binding also prevents  α-amylase expression.

e) GA-MYB can therefore be defined as a primary or early response gene, while the α-amylase gene is a secondary or late response gene

f) The secretory pathway requires GA stimulation of the calcium-calmodulin– independent pathway.

Which of the following statements is incorrect about this transcriptional gene?

TLS Online TPP Program

#Question id: 11722

#Unit 6. System Physiology – Plant

The induction of GA-MYB and α-amylase mRNA by GA3, the production of GA-MYB  and  α-amylase mRNA has shown in graph, which of the following is the correct representation relation between these two mRNA .

TLS Online TPP Program

#Question id: 11723

#Unit 6. System Physiology – Plant

During the α-amylase secretion pathway, a mutation will be found in the sequence of gibberellic acid response element (GARE) the what will caused;

TLS Online TPP Program

#Question id: 11724

#Unit 6. System Physiology – Plant

At the subcellular level, starch is stored in amyloplasts in the endosperm of cereals. Two enzymes responsible for initiating starch degradation are α- and β-amylase

a) α-Amylase hydrolyzes starch chains internally to produce oligosaccharides consisting of α(1,4)-linked glucose residues

b) β-Amylase degrades these oligosaccharides from the ends to produce maltose, a disaccharide

c) α-Amylase degrades these oligosaccharides from the ends to produce maltose, a disaccharide

d)  α-Amylase hydrolyzes starch chains internally to produce oligosaccharides consisting of  β(1,4)-linked glucose residues

e) β-Amylase also converts maltose to glucose

Which of the following combination from the above statements is correct?

TLS Online TPP Program

#Question id: 11725

#Unit 6. System Physiology – Plant

Given some statements about the roles of phytochrome and gibberellin in shade avoidance response;

A) In direct sunlight, red light predominates and the Pfr form of phytochrome moves into the nucleus.

B) Under a plant canopy, the light is enriched in far-red wavelengths. Phytochrome is in the inactive Pr form.

C) As PIF proteins accumulate, PIF-induced gene expression increases, promoting stem elongation.

D) Phy causes the turnover of PIF proteins, which act as negative regulators of  photomorphogenesis

E) In the absence of Pfr, PIFs are not degraded, the sensitivity to gibberellin increases, causing the degradation of DELLA repressors

F) As a result, DELLA repressors bind PIF and prevent the transcription of PIF-regulated gene, In the absence of PIF-induced gene expression, stem growth is limited.

Find out the correct sequence of these responses.