TLS Online TPP Program

#Question id: 906


Which of the following is least likely to result in protein denaturation?

#Unit 1. Molecules and their Interaction Relevant to Biology
  1. Altering net charge by changing pH

  2. Changing the salt concentration

  3. Disruption of weak interactions by boiling

  4. Exposure to detergents

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TLS Online TPP Program

#Question id: 33907

#Unit 2. Cellular Organization

A circular DNA molecule with 580,070 bp. Calculate the molecular weight and contour length (when relaxed) of this molecule. What is Lko for the Mycoplasma chromosome? If σ=-  0.06, what is Lk?

TLS Online TPP Program

#Question id: 33908

#Unit 2. Cellular Organization

How is the linking number altered if one DNA strand is broken

TLS Online TPP Program

#Question id: 33909

#Unit 2. Cellular Organization

What is the Lk of a 5,000 bp circular duplex DNA molecule with a nick in one strand?

TLS Online TPP Program

#Question id: 33910

#Unit 2. Cellular Organization

What is the Lk of a 5,000 bp circular duplex DNA molecule after eight enzymatic turnovers by a single molecule of DNA gyrase in the presence of ATP?

TLS Online TPP Program

#Question id: 33911

#Unit 2. Cellular Organization

What is the Lk of a 5,000 bp circular duplex DNA molecule after eight enzymatic turnovers by a single molecule of DNA gyrase in the presence of ATP followed by 8 enzymatic turnovers by a single molecule of bacterial type I topoisomerase

TLS Online TPP Program

#Question id: 33912

#Unit 2. Cellular Organization

A. When a DNA molecule is negatively supercoiled, it is underwound. 
B. Any structural change involving DNA strand separation becomes more likely in negatively supercoiled DNA. 
C. Negative supercoiling makes establishment of left-handed Z-DNA in a segment of DNA 
D. The formation of plectonemic or solenoidal supercoils is favored as a means of negative supercoiling.

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