TLS Online TPP Program

#Question id: 910


Experiments on denaturation and renaturation after the reduction and reoxidation of the —S—S— bonds in the enzyme ribonuclease (RNase) have shown that:

#Unit 1. Structure and Function of Biomolecules
  1. folding of denatured RNase into the native, active conformation, requires the input of energy in the form of heat.

  2. native ribonuclease does not have a unique secondary and tertiary structure.

  3. the completely unfolded enzyme, with all —S—S— bonds broken, is still enzymatically active.

  4. the primary sequence of RNase is sufficient to determine its specific secondary and tertiary structure.

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TLS Online TPP Program

#Question id: 650

#Unit 1. Structure and Function of Biomolecules

On the basis of hypothetical binding curve given below, which of the following statement is INCORRECT

TLS Online TPP Program

#Question id: 651

#Unit 1. Structure and Function of Biomolecules

Consider an enzyme catalysed reaction treated with inhibitor. Enzyme kinetic was analysed with Eadie Hofstee equation. A plot of V0 versus V0/[S] for an enzyme-catalyzed reaction is shown below. The control curve was obtained in the absence of inhibitor. Which effect is shown on the enzyme activity when an inhibitor is added to the reaction mixture?

TLS Online TPP Program

#Question id: 652

#Unit 1. Structure and Function of Biomolecules

Two proteins, X and Y, bind to the same ligand, A, with the binding curves shown below. Which of the statement is correct for the graph analysing two proteins?

TLS Online TPP Program

#Question id: 653

#Unit 1. Structure and Function of Biomolecules

For the enzyme-catalyzed reaction S ~ P, Keq = 5, which of the following curves best represents the change in [S] that occurs with time when S is added to a solution containing the appropriate enzyme?

TLS Online TPP Program

#Question id: 653

#Unit 1. Structure and Function of Biomolecules

For the enzyme-catalyzed reaction S ~ P, Keq = 5, which of the following curves best represents the change in [S] that occurs with time when S is added to a solution containing the appropriate enzyme?

TLS Online TPP Program

#Question id: 654

#Unit 1. Structure and Function of Biomolecules

Which pair represents different conformations?