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TLS Online TPP Program

#Question id: 12180

Following statements are regarding to cotransport.
A. The two forces constituting the electrochemical gradient—the membrane electric potential and the ion concentration gradient—may act in the same or opposite directions.
B. Cotransporters use the energy released by movement of an ion (usually H+ or Na+) down its electrochemical gradient to power the import or export of a small molecule or different ion against its concentration gradient.
C. Cotransporters use the energy released by movement of an ion (usually H+ or Na+) down its electrochemical gradient to power the import or export of a small molecule or different ion down its concentration gradient.
D. The cells lining the small intestine and kidney tubules contain symporters that couple the energetically favorable entry of Na+ to the import of glucose against its concentration gradient.
Which of the following is incorrect?

#Unit 2. Cellular Organization
  1. A and B
  2. C and D
  3. C only
  4. D only
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TLS Online TPP Program

#Question id: 220

#Unit 1. Molecules and their Interaction Relevant to Biology

Which structure is not a part of folate?
TLS Online TPP Program

#Question id: 31295

#Unit 5. Developmental Biology

Which of the following cells become Nieuwkoop center?
TLS Online TPP Program

#Question id: 18783

#Unit 13. Methods in Biology

Sensitivity of various immunoassays are given match them correctly

Assay

Sensitivity∗ (g antibody/ml)

a) Mancini radial immunodiffusion

i) 2

b) Ouchterlony double immunodiffusion

ii) 20–200

c) Immunoelectrophoresis

iii) 20–200

d) Rocket electrophoresis

iv) 10–50

TLS Online TPP Program

#Question id: 13088

#Unit 13. Methods in Biology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
 
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
 
What is the Colony 1’s plasmid is;
TLS Online TPP Program

#Question id: 2736

#Unit 2. Cellular Organization

Which histone molecule produces novel binding sites for protein components of the kinetochore?