Following statements are regarding to regulation of S phase and mitotic cyclin levels in budding yeast.

A. In late anaphase, the anaphase-promoting complex (APC/C) ubiquitinylates S phase and mitotic cyclins.

B. In late anaphase, the anaphase-promoting complex (APC/C) phosphorylates S phase and mitotic cyclins.

C. During exit from mitosis and G1, Cdh1 is dephosphorylated and active; during S phase and mitosis, Cdh1 is phosphorylated and dissociates from APC/C, and APC/C becomes inactive.

D. During exit from mitosis and G1, Cdh1 is phosphorylated and active; during S phase and mitosis, Cdh1 is dephosphorylated and dissociates from APC/C, and APC/C becomes inactive.

E. The G1/S phase CDKs, which themselves are not APC/CCdh1 substrates, phosphorylate Cdh1 at the G1–S phase transition.

F. The G1/S phase CDKs, which themselves are not APC/CCdh1 substrates, ubiquitinylates Cdh1 at the G1–S phase transition.

Which of the following combination is correct?

TLS Online TPP Program

#Question id: 18777

#Unit 13. Methods in Biology

which of the following technique using antibodies labelled with fluorescent molecules can be used to visualize antigen on or within cells.

TLS Online TPP Program

#Question id: 18778

#Unit 13. Methods in Biology

which of the following technique provides an unusually powerful technology for the quantitative analysis and sorting of cell populations labelled with one or more fluorescent antibodies.

TLS Online TPP Program

#Question id: 18779

#Unit 13. Methods in Biology

Flow-cytometric analysis for the detection and measurement of subpopulations of leukocytes, including those of leukaemia, is usually performed using monoclonal antibodies. Why is this the case?

Choose whether each of the following statements is true 

a) Indirect immunofluorescence is a more sensitive technique than direct immunofluorescence

b) Most antigens induce a polyclonal response. 

c) For precipitation to occur, both antigen and antibody must be multivalent.

d) ELISA tests using chemiluminescence are more sensitive than chromogenic ones and precipitation tests are more sensitive than agglutination tests

TLS Online TPP Program

#Question id: 18780

#Unit 13. Methods in Biology

Which of the following does not participate in the formation of antigen-antibody complexes?

TLS Online TPP Program

#Question id: 18781

#Unit 13. Methods in Biology

In which of the following case a large lattice is formed?

TLS Online TPP Program

#Question id: 18782

#Unit 13. Methods in Biology

The concentration of a small amount antibodies 2 μg antibody/ml can be detected