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TLS Online TPP Program

#Question id: 12187

How cells know whether sister kinetochores are properly attached to the mitotic spindle?

#Unit 2. Cellular Organization
  1. When sister chromatids are correctly attached to microtubules, their kinetochores are under tension. Mitotic spindle attached to the kinetochores pull at them, and the cohesin molecules that hold the sister chromatids together withstand these forces, creating tension at kinetochores.
  2. When sister chromatids are correctly attached to microtubules, their kinetochores are not under tension. One kinetochore attaches to microtubules emanating from two opposite spindle poles.
  3. When sister chromatids are correctly attached to microtubules, their kinetochores are not under tension. Both sister kinetochores attach to microtubules emanating from the same spindle pole.
  4. When sister chromatids are correctly attached to microtubules, their kinetochores are not under tension. Only one of the two sister kinetochores attaches to microtubules.
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TLS Online TPP Program

#Question id: 3814

#Unit 3. Fundamental Processes

The mechanism of DNA replication is studied in an E. coli replication fork.

Which is a property of RNA primers in an E. coli replication fork?
TLS Online TPP Program

#Question id: 3815

#Unit 3. Fundamental Processes

Use the figure to answer the following question. A space probe returns with a culture of a microorganism found on a distant planet. Analysis shows that it is a carbon-based life-form that has DNA. You grow the cells in 15N medium for several generations and then transfer them to 14N medium. Which pattern in the figure would you expect if the DNA was replicated in a conservative manner?
TLS Online TPP Program

#Question id: 3816

#Unit 3. Fundamental Processes

The mechanism of DNA replication is studied in an E. coli replication fork. Which is a characteristic of an E. coli replication fork and a eukaryotic replication fork?
TLS Online TPP Program

#Question id: 3817

#Unit 3. Fundamental Processes

DNA replication is studied in a newly discovered bacterium. It takes 30 min for the bacterium to complete a round of replication at 37oC. A Meselson-Stahl-type experiment was performed. The bacteria were grown for several generations in 14N-medium and then switched to 15N-medium. The DNA molecules were then analyzed.

If the mechanism of DNA replication in this bacterium were dispersive, what results would be found when the double-stranded DNA was analyzed in a CsCl gradient after two generations in 15N-medium?
TLS Online TPP Program

#Question id: 3818

#Unit 3. Fundamental Processes

DNA replication is studied in a newly discovered bacterium. It takes 30 min for the bacterium to complete a round of replication at 37oC. A Meselson-Stahl-type experiment was performed. The bacteria were grown for several generations in 14N-medium and then switched to 15N-medium. The DNA molecules were then analyzed.

If the mechanism of DNA replication in this bacterium were semi-conservative, what results would be found when the double-stranded DNA was analyzed in a CsCl density gradient after three generations in 15N-medium?
TLS Online TPP Program

#Question id: 3819

#Unit 3. Fundamental Processes

DNA replication is studied in a newly discovered bacterium. It takes 30 min for the bacterium to complete a round of replication at 37oC. A Meselson-Stahl-type experiment was performed. The bacteria were grown for several generations in 14N-medium and then switched to 15N-medium. The DNA molecules were then analyzed.

After one generation in 15N-medium, the DNA is denatured and analyzed in a CsCl gradient. If the single-stranded DNA forms two bands, one with heavy density and one with light density, what conclusion could be drawn about the mechanism of DNA replication in this bacterium?