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TLS Online TPP Program

#Question id: 12212

A 71-year-old man with upper abdominal pain and blood in the stool takes NSAIDS for the pain and washes it down with whiskey. Pentagastrin administration produced lower than predicted levels of gastric acid secretion. Secretion of which of the following substances is most likely to be diminished in this patient with gastritis?

#Unit 7. System Physiology – Animal

Intrinsic factor
Ptyalin
Rennin
Trypsin

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TLS Online TPP Program

#Question id: 4546

#Unit 3. Fundamental Processes

In eukaryotes, transcriptional regulators are not typically controlled at the level of DNA binding (although there are exceptions). Regulators are instead usually controlled in one of the following two basic ways.

I. Unmasking an Activating Region

II. Transport into and out of the Nucleus

III. Mediated through proteolysis of an inhibitor or tethering region or by allosteric changes.

IV. Also is itself (or recruits) a deacetylase,

TLS Online TPP Program

#Question id: 1501

#Unit 4. Cell Communication and Cell Signaling

When a culture of rodent cells is infected with a DNA tumor virus whose gene products bind to p53 and RB, all the cells take on characteristics of malignancy. If the viral gene coding the p53-binding protein is mutated, but that coding for the RB-binding protein is not mutated, how will infected cells respond?

TLS Online TPP Program

#Question id: 13090

#Unit 13. Methods in Biology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

What is the Colony 3’s plasmid is;

TLS Online TPP Program

#Question id: 28162

#Unit 13. Methods in Biology