The following test results were obtained: urine flow rate = 2.0 ml/min; urine inulin concentration = 60 mg/ml; plasma inulin concentration = 2 mg/ml; urine potassium concentration = 20 μmol/ml; plasma potassium concentration = 4.0 μmol/ml; urine osmolarity = 150 mOsm/L; and plasma osmolarity = 300 mOsm/L. What is the approximate GFR?

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#Question id: 19888

#Unit 13. Methods in Biology

There are some typical enzyme used in the manipulation of nucleic acid;

ENZYMES	USE IN NUCLEIC ACID MANIPULATION
A) klenow	i) DNA-dependent RNA polymerase
B) T4 DNA polymerase	ii) Single-strand specific nuclease
C) Taq DNA polymerase	iii) DNA pol I lacks 5’→ 3’ exonuclease activity
D) T7 RNA polymerase	iv) Thermostable DNA polymerase used in PCR
E) S1 nuclease	v) Lack  5’→ 3’ exonuclease activity

TLS Online TPP Program

#Question id: 19917

#Unit 13. Methods in Biology

Antigen-Antibody interactions are__

TLS Online TPP Program

#Question id: 19918

#Unit 13. Methods in Biology

Affinity constant (Ka) in between the antigen-antibody interaction can be analysed by which method?

TLS Online TPP Program

#Question id: 19919

#Unit 13. Methods in Biology

Scatchard plots are based on repeated equilibrium dialyses with a constant concentration of antibody and varying concentration of ligand. Which plots correctly verify the Scatchard equation that determined by equilibrium dialysis?

TLS Online TPP Program

#Question id: 19920

#Unit 13. Methods in Biology

Ag-Ab reactions are highly specific, in some cases antibody elicited by one antigen and with unrelated antigen also, Such two different antigens share an identical or very similar epitope then it consider 

TLS Online TPP Program

#Question id: 20000

#Unit 13. Methods in Biology

Example of Cross-reactivity

a) Dominant and recessive gene

b) Autoimmune disease

c) Blood group mismatch