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TLS Online TPP Program

#Question id: 12904

Match the following:
1. Southern Blotting                               i. Melting temperature of DNA strand
2. DNA microchips                                   ii.oligonucleotide matching to DNA sample
3. ASO-PCR                                                 iii.DNA hybridization to oligo arrays
4. DGGE                                                        iv. labeled probe hybridization to DNA  

#Unit 13. Methods in Biology
  1. 1-iv,2-iii,3-ii,4-i
  2. 1-iii,2-iv,3-i,4-ii
  3. 1-iv,2-ii,3-i,4-iii
  4. 1-i, 2-iii, 3-iv, 4-ii
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TLS Online TPP Program

#Question id: 23162

#Unit 13. Methods in Biology

Match the fluorescent dyes in column I with emission light in column II
Column I                                                                          Column II
1. Texas                                                                            a. far red
2. Fluorescein                                                                   b. red
3. Rhodamine                                                                   c. blue 
4. Cy5                                                                               d. green
Which of the following option comprises all correct matches?
TLS Online TPP Program

#Question id: 23163

#Unit 13. Methods in Biology

Out of the given below alexa dyes which one will emit in red light range?
TLS Online TPP Program

#Question id: 23164

#Unit 13. Methods in Biology

Out of the given below alexa dyes which one will emit in Blue light range?
TLS Online TPP Program

#Question id: 23165

#Unit 13. Methods in Biology

For visualizing two proteins which are localized in nucleus through fluorescence microscopy which two sets of dyes can be best used for clear visual?
TLS Online TPP Program

#Question id: 23166

#Unit 13. Methods in Biology

To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.
1. FITC
2. GFP
3. Fluorescein
4. Alexa 568
Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?
TLS Online TPP Program

#Question id: 23167

#Unit 13. Methods in Biology

A secondary antibody can be generated by immunizing a goat with antibody which segment that is common to all rabbit IgG antibodies called goat anti-rabbit.