According to the diagram, arrange the steps in correct order: 

1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 

2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 

3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).

 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.

#Question id: 13213

#Unit 6. System Physiology – Plant

#Question id: 2963

#Unit 2. Cellular Organization

Eukaryotic chromosomes are composed of which of the following macromolecules?

#Question id: 10912

#Unit 6. System Physiology – Plant

Velocities for transport in the phloem are measured by using a technique which detect water flow;

#Question id: 4101

#Unit 3. Fundamental Processes

In order to ensure that only fully processed mature mRNAs are allowed to be exported to cytosol, pre-mRNAs associated with snRNPs are retained in the nucleus. To demonstrate this, an experiment was performed where a gene coding a pre-mRNA with a single intron was mutated either at the 5’ or 3’ splice sites or both the splice sites.

Given below are a few possible outcomes, Choose NOT FEASIBLE OUTCOME

A. Pre-mRNA having mutation at both the splice sites will be retained in the nucleus because of the presence of bound snRNPs.

B. Pre-mRNA having mutation at both the splice sites will be exported to cytosol because of the absence of bound snRNPs.

C. Pre-mRNA mutated at either 3’ or 5’ splice sites will be retained in the nucleus because of the presence of bound snRNPs.

D. Pre-mRNA mutated at either 3’ or 5’ splice sites will be exported to cytosol because of the absence of bound snRNPs.

#Question id: 11113

#Unit 7. System Physiology – Animal

Exchange of both oxygen and carbon dioxide through the respiratory membrane occurs by: