Name the enzyme which separates the double-helical DNA into single strands.

TLS Online TPP Program

#Question id: 18612

#Unit 13. Methods in Biology

A PCR product observed after 6 min. the product will be

a) 100% long product

b) 100% accurate product

c) 50% long product

d) 50% accurate product

TLS Online TPP Program

#Question id: 18613

#Unit 13. Methods in Biology

The size of a PCR product is mainly determined 

TLS Online TPP Program

#Question id: 18614

#Unit 13. Methods in Biology

The primer length affects the efficiency rate of PCR, this rate decreases as the primer length

TLS Online TPP Program

#Question id: 18615

#Unit 13. Methods in Biology

PCR efficiency is influenced by several factors, the efficiency declines due to

a) efficiency of amplification declines with an increase in the length of the target sequence.

b) Efficiency of PCR is affected by primer length; it declines if the primers used for PCR arc too long.

c) a temperature higher than the ideal annealing temperature reduces PCR efficiency.

d)  primers used for PCR have complementary regions, dimers will be formed, and PCR efficiency will decline.

TLS Online TPP Program

#Question id: 18616

#Unit 13. Methods in Biology

In a PCR higher annealing temperature along with7-dcaza guanosine triphosphate (7-deaza OTP) used in place of GTP this indicate

TLS Online TPP Program

#Question id: 18617

#Unit 13. Methods in Biology

Both the metal ion used in PCR have different functions