Choose the correct order for the cloning process.

1. The production of cDNA is carried out using an enzyme termed Reverse transcriptase.

2. mRNA cannot be used directly in cloning.

3. It is possible to synthesis complementary DNA molecule to all the mRNA from the selected tissue.

4. The cDNA may be inserted into vectors and then cloned.

TLS Online TPP Program

#Question id: 10188

#Unit 1. Molecules and their Interaction Relevant to Biology

Kinetic Isotope Effects (KIEs) are used to determine

A. reaction mechanisms

B. rate limiting steps

C. transition states

Which of the following is correct combination

TLS Online TPP Program

#Question id: 10189

#Unit 1. Molecules and their Interaction Relevant to Biology

A very common isotope substitution is when hydrogen is replaced by deuterium. This is known as a deuterium effect and is expressed by the ratio kH/kD (as explained above). Normal KIEs for the deuterium effect

TLS Online TPP Program

#Question id: 10190

#Unit 1. Molecules and their Interaction Relevant to Biology

Arrange the protium, deuterium and tritium water in increasing order of molarity. Suppose density 1g/ml.

TLS Online TPP Program

#Question id: 10191

#Unit 1. Molecules and their Interaction Relevant to Biology

Statements are given as

A. Primary kinetic isotope effects are rate changes due to isotopic substitution at a site of bond breaking in the rate determining step of a reaction.

B. Solvent shows primary kinetic isotope effect

C. Secondary kinetic isotope effects are rate changes due to isotopic substitutions at a site other than the bond breaking site in the rate determining step of the reaction.

D. Solvent shows primary kinetic isotope effect and secondary kinetic effect as well

Which of the following is correct combination of statements

TLS Online TPP Program

#Question id: 10257

#Unit 1. Molecules and their Interaction Relevant to Biology

Lattice energy is inversely proportional to sum of radii of

TLS Online TPP Program

#Question id: 10258

#Unit 1. Molecules and their Interaction Relevant to Biology

Arrange in the decreasing order of Lattice Energy (kj/mol)