Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

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#Question id: 2368

#Unit 2. Cellular Organization

 Which of these produce most of the ATP in aerobic cellular respiration?

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#Question id: 32300

#Unit 1. Molecules and their Interaction Relevant to Biology

Identify the structure shown

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#Question id: 2803

#Unit 2. Cellular Organization

A human cell contains 3X10⁹ bp per set of chromosomes.

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#Question id: 11080

#Unit 7. System Physiology – Animal

The O2-CO2 diagram here shows a ventilation-perfusion ratio line for the normal lung. Which of the following best describes the effect of decreasing ventilation-perfusion ratio on the alveolar Po2 and Pco2?

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#Question id: 2547

#Unit 2. Cellular Organization

Suppose a cell has the following molecules and structures: enzymes, DNA, ribosomes, plasma membrane, and mitochondria. It could be a cell from ________.