Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

#Question id: 3426

#Unit 9. Evolution & Diversity of Life Forms

Fitness of three different genotypes is given in population I and II, under natural selection which of the following statement are correct?

#Question id: 3427

#Unit 9. Evolution & Diversity of Life Forms

Natural selection can alter the frequency of distribution allele and heritable trait in different following way

A- Accumulation of those mutation that increase fitness of population in changing environment

B- Check accumulation of mutation that lower the fitness of species in unchanging environment

c- occur when population is subjected to divergent selection

#Question id: 3428

#Unit 9. Evolution & Diversity of Life Forms

In a sample from an African population, the frequency of blood group allele IA, IB and IO were 0.30, 0.20 and 0.50 respectively. What is expected frequency of A and B blood group respectively when population size 1000?

#Question id: 3429

#Unit 9. Evolution & Diversity of Life Forms

Which of the following is a behavioral pattern that results from a proximate cause?

#Question id: 3430

#Unit 9. Evolution & Diversity of Life Forms

The proximate causes of behavior are interactions with the environment, but behavior is ultimately shaped by

#Question id: 3431

#Unit 9. Evolution & Diversity of Life Forms

In the territorial behavior of the stickleback fish, the red belly of one male elicits attack from another male by functioning as