Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

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#Question id: 14754

#Unit 6. System Physiology – Plant

Plant cells have several internal compartments, each of which can differ in its ionic composition. The cytosol and the vacuole are the most important intracellular compartments that determine the ionic relations of plant cells, Ion concentrations in the cytosol and the vacuole are controlled by passive (dashed arrows) and active (solid arrows) transport processes

  

Which of the following statement INCORRECT from the above figure;

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#Question id: 14755

#Unit 6. System Physiology – Plant

Any active transport mechanism that results in the movement of a net electric charge will tend to move the membrane potential away from the value predicted by the Goldman equation. Such a transport mechanism is called

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#Question id: 14756

#Unit 5. Developmental Biology

A series of mitotic divisions whereby the enormous volume of egg cytoplasm is divided into numerous smaller, nucleated cells. These cleavage-stage cells are called as:

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#Question id: 14757

#Unit 5. Developmental Biology

Which of the following events are responsible for transition from fertilization to cleavage ?

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#Question id: 14758

#Unit 5. Developmental Biology

If contractile ring is disrupted during cleavage, which of the following process stop?

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#Question id: 14759

#Unit 5. Developmental Biology

Which of the following mechanical agent creates cleavage furrow during cleavage?