Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

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#Question id: 4184

#Unit 3. Fundamental Processes

Translation takes place:

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#Question id: 10847

#Unit 10. Ecological Principles

In the stomachs of ruminants, such as cows, certain microbes digest cellulose, a material the cow cannot digest itself. This interaction between cows and microbes represents an example of

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#Question id: 31232

#Unit 2. Cellular Organization

Condensin association with chromosomes is facilitated by mitotic CDKs and Aurora B kinase, which phosphorylates 

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#Question id: 12367

#Unit 1. Molecules and their Interaction Relevant to Biology

RNA contains ribose sugar. What could happen due the presence of extra hydroxyl group?

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#Question id: 1180

#Unit 4. Cell Communication and Cell Signaling

In which of the following double-mutant flies will R7 photoreceptors develop normally?