Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

In blue white screening insertional activation of Beta-D-galactosidase will give rise to

TLS Online TPP Program

#Question id: 23257

#Unit 13. Methods in Biology

Centromeric probes are useful in identifying 

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#Question id: 11292

#Unit 10. Ecological Principles

In an pond ecosystem the _____ is always greater than the _____ .

(a) number of primary producers . . . number of secondary consumers

(b) biomass of secondary consumers . . . biomass of primary consumer

(c) assimilation efficiency of primary consumers . . . assimilation efficiency of secondary consumers

(d) biomass of primary producers . . . biomass of primary consumers

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#Question id: 15176

#Unit 2. Cellular Organization

Some Psychrophiles which can grow at maximum temperature of about 35°C, they are called as 

A. Obligatory psychrophiles

B. Facultative psychrophiles

C. Psychrotrophs

D. Mesophiles

Which combination given below is correct.

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#Question id: 19183

#Unit 13. Methods in Biology

Reverse transciptase PCR is also carried out at times. Which of the statement is true?

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#Question id: 29464

#Unit 6. System Physiology – Plant

The auxin receptor is composed of two proteins

a. SCF complex component TIR1 

b. repressor protein AUX/IAA

c. repressor protein component TIR1 

d. SCF complex component AUX/IAA

Which of the following is correct