Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Arrange in the order of their increasing capacity:

TLS Online TPP Program

#Question id: 10411

#Unit 6. System Physiology – Plant

Phytochrome chromophore which absorbs light known as Phytochromobilin , that are synthesized in

TLS Online TPP Program

#Question id: 10412

#Unit 6. System Physiology – Plant

Red and far-red lights are perceived by plants through various photoreceptors including phytochromes. The red/far-red reversibility of seed germination was due to the-

a) photoreversibility of phytochrome

b) presence of red and far-red light at different fluence rate

c) Conversion of Pr to Pfr form through the effect of red light

d) equal proportion of red and far red light at same fluence rate

Which of the following combinations is correct?

TLS Online TPP Program

#Question id: 10413

#Unit 6. System Physiology – Plant

Following are certain statements are related to the effect of red and far-red light;

a) under a dense canopy much of the red light is absorbed by plant pigments, resulting in transmittance of mostly far-red light

b) Pr (red) has highest photon flux at 680 nm while Pfr (far-red) has highest photon flux at 730nm

c) Pfr far-red light–absorbing form, which is considered to be the physiologically active form of phytochrome

d) R:FR ratio is strongly affected by the presence of a canopy because chlorophyll absorbs far-red but not red light

Which one of the combinations of above statements is incorrect?

TLS Online TPP Program

#Question id: 10413

#Unit 1. Molecules and their Interaction Relevant to Biology

Following are certain statements are related to the effect of red and far-red light;

a) under a dense canopy much of the red light is absorbed by plant pigments, resulting in transmittance of mostly far-red light

b) Pr (red) has highest photon flux at 680 nm while Pfr (far-red) has highest photon flux at 730nm

c) Pfr far-red light–absorbing form, which is considered to be the physiologically active form of phytochrome

d) R:FR ratio is strongly affected by the presence of a canopy because chlorophyll absorbs far-red but not red light

Which one of the combinations of above statements is incorrect?

TLS Online TPP Program

#Question id: 10414

#Unit 6. System Physiology – Plant

Plants have evolved with multiple photoreceptors, Phytochrome is one of them. Which of the following statements is incorrect about these photoreceptors?

TLS Online TPP Program

#Question id: 10415

#Unit 6. System Physiology – Plant

Bacterial phytochromes are light-dependent kinases Which of the following classes of kinases does it represent?