Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Arrange in the order of their increasing capacity:

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#Question id: 9156

#Unit 9. Diversity of Life Forms

Generalized global air circulation and precipitation patterns are caused by ________.

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#Question id: 7206

#Unit 5. Developmental Biology

Which of the following statements explains the dominant mutation of Pmar1 results in a vegetalized embryo is due to:

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#Question id: 26582

#Unit 3. Fundamental Processes

Select the incorrect statement

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#Question id: 9189

#Unit 9. Diversity of Life Forms

A plant will recognize a pathogenic invader

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#Question id: 34147

#Unit 7. System Physiology – Animal

Which of the following statements not CORRECT regarding the mechanism and reproductive role of prolactin during lactation?