Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Arrange in the order of their increasing capacity:

TLS Online TPP Program

#Question id: 15249

#Unit 2. Cellular Organization

Halophiles have adapted so completely to ____________ conditions that they require high levels of ___________ to grow.

TLS Online TPP Program

#Question id: 15250

#Unit 2. Cellular Organization

Osmotolerant microorganisms can survive at extreme osmotic pressure because

TLS Online TPP Program

#Question id: 15251

#Unit 2. Cellular Organization

The alga Dunaliella viridis tolerates high concentrations of

TLS Online TPP Program

#Question id: 15252

#Unit 2. Cellular Organization

A process in which a suspension of microbes is quickly frozen at temperatures ranging from - 54°C to -72°C, and the water is removed by a high vacuum (sublimation) 

TLS Online TPP Program

#Question id: 15253

#Unit 2. Cellular Organization

The water activity in foods that have high levels of salt or sugar is

TLS Online TPP Program

#Question id: 15254

#Unit 2. Cellular Organization

For growth, most bacteria require water activity levels above