Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Arrange in the order of their increasing capacity:

#Question id: 11607

#Unit 6. System Physiology – Plant

How the active gibberellin GA1 is inactived by?

a)  By hydroxylation remove GA20 from the biosynthetic pathway by converting it to GA1

b) conversion to GA8 by a hydroxylation on carbon 2

c) By hydroxylation remove GA20 from the biosynthetic pathway by converting it to GA29

#Question id: 11608

#Unit 6. System Physiology – Plant

Which of the following  growth hormone  promote stem growth and were discovered in relation to the “foolish seedling disease” of rice?

#Question id: 11609

#Unit 6. System Physiology – Plant

All of gibberellins are tetracyclic diterpenoid acids, but only a few of which, have intrinsic biologically active gibberellins these are__

#Question id: 11610

#Unit 6. System Physiology – Plant

Environmental factors such as photoperiod and temperature can alter the levels of active gibberellins by affecting gene transcription for specific steps in the biosynthetic pathway such as;

a) Promotion of germination by light has been shown to be due to increases in GA1 levels resulting from a light-induced increase in the transcription of the gene for GA3ox, which converts GA20 to GA1

b) level of GA20-oxidase occurs in the highest amount in shoot tips and elongating stems, is increased under long-day conditions.  The fact that GA 20-oxidase is the enzyme that converts GA53 to GA20

c) Giving cold treatment  of Thlaspiarvense and a return to high temperatures, the ent-kaurenoic acid is converted to GA9, the most active gibberellin for stimulating the flowering response

d) Potato plants overexpressing the GA20ox gene showed tuberization, whereas transformation with the antisense gene for GA20ox inhibited tuberization

Which of the following from the given above statements is correct?

#Question id: 11611

#Unit 6. System Physiology – Plant

Given below are names of phytohormones in column I and their associated features/effects/ functions in column II.

Which of the following combination from the above statements is  correct? 

#Question id: 11612

#Unit 6. System Physiology – Plant

AMO-1618, which blocks GA biosynthesis at the cyclization step, what would be the A, B and C represents. where, the control shows no inhibitor;