Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Arrange in the order of their increasing capacity:

#Question id: 1230

#Unit 4. Cell Communication and Cell Signaling

Which of the following events occur(s) following the binding of a ligand to a G protein–coupled receptor that triggers release of Ca2+?

a. activation of protein kinase A by IP3

b. phospholipase cleavage of PIP2 to IP3 and DAG

c. release of DAG into the cytosol

d. IP3-mediated opening of Ca2+ channels in the ER membrane

#Question id: 1231

#Unit 4. Cell Communication and Cell Signaling

Arrange the following events in the proper order in which they occur during transduction of the TGFβ signal:

a. association of Smad3 and Smad4  

b. phosphorylation of TGFβ receptor I by TGFβ receptor II            

c. phosphorylation of Smad3 by TGFβ receptor I     

d. nuclear import of Smad3   

e. binding of TGFβ to TGFβ receptor II  

#Question id: 1232

#Unit 4. Cell Communication and Cell Signaling

Which of the following mutations might confer a competitive advantage to an athlete by raising his or her hematocrit?

a. excess production of erythropoietin                       

b. decreased production of erythropoietin

c. EpoR that cannot bind STAT5                  

d. EpoR that cannot bind SHP1

e. EpoR that cannot bind JAK2

#Question id: 1232

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of the following mutations might confer a competitive advantage to an athlete by raising his or her hematocrit?

a. excess production of erythropoietin                       

b. decreased production of erythropoietin

c. EpoR that cannot bind STAT5                  

d. EpoR that cannot bind SHP1

e. EpoR that cannot bind JAK2

#Question id: 1233

#Unit 4. Cell Communication and Cell Signaling

Transphosphorylation (cross-phosphorylation) of receptor tyrosine kinases

a. inhibits catalytic activity.

b. promotes transphosphorylation of additional tyrosine residues.

c. triggers release of the ligand.

d. generates binding sites for signaling molecules.

#Question id: 1234

#Unit 4. Cell Communication and Cell Signaling

Which of the following mutations would likely have a similar cancer-promoting effect as the RasD mutation?

a. mutation in Grb2 so that it cannot bind Sos

b. mutation in Sos so that it binds Ras independent of Grb2

c. mutation in GAP so that it cannot bind Ras

d. mutation in EGF receptor so that it binds GRB2 independent of EGF