Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?

TLS Online TPP Program

#Question id: 12998

#Unit 1. Molecules and their Interaction Relevant to Biology

Match with appropriate bonding pattern

TLS Online TPP Program

#Question id: 12999

#Unit 1. Molecules and their Interaction Relevant to Biology

Proline and glycine both disturb the symmetry of α-helix which of the following are true regarding to these two amino acids

a) proline changes the orientation of hetropolymeric α-helix hence proline can be placed only as 1st amino acid residue near to C-terminus 

b) proline changes the orientation of polymeric α-helix hence proline can be placed only as 1st amino acid residue near to N-terminus

c) glycine disrupts the heteropolymeric α-helix by changing the helicity.

d) homopolymer of proline and glycine can form stable helix. 

TLS Online TPP Program

#Question id: 13000

#Unit 1. Molecules and their Interaction Relevant to Biology

Identify the correct statement regarding the structure

TLS Online TPP Program

#Question id: 13001

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of the following is incorrect 

a) antiparallel β sheets are frequently found and they are quite more stable because of more no. hydrogen bond presence and linear H bonding pattern.

b) antiparallel β sheets are frequently found and they are quite more stable because of more no. hydrogen bond presence and having distorted H bonding pattern.

c) parallel β sheets are relatively less stable and mostly found in core of protein.

d) parallel β sheets are relatively more stable and mostly found in core of protein.

TLS Online TPP Program

#Question id: 13002

#Unit 3. Fundamental Processes

Where in a eukaryotic cell do you think you would find each of the following proteins residing when it is actively performing its function. Be as specific as you can in terms of subcellular location.

Match the following with the correct subcellular location;

I) Polymerases	a) In the cytoplasm
II) Ribosomal proteins	b) Inside the plasma membrane.
III) DNA ligase	c) In the mitochondrial matrix
IV) protein that allows ions to pass in and out of the cell	d) In the nucleus of cells
V) Activator protein	e) Inside the nuclear membrane
VI) an enzyme in the Krebs/TCA cycle
VII) a protein that forms a channel through which mRNAs can be exported into the cytoplasm

TLS Online TPP Program

#Question id: 13003

#Unit 8. Inheritance Biology

If 200 meiocytes with AaBb and   80 meiocytes  undergoes to single crossing over between  gene A and B. What will be the chance of crossing over and what is the recombination frequency of the gametes?