Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the statement is worng? Aminopetrin………s

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#Question id: 28393

#Unit 5. Developmental Biology

The mechanism for this “bottom-up” specification of the frog embryo resides in a set of mRNAs that are tethered to the vegetal cortex. This includes the mRNA such as___

TLS Online TPP Program

#Question id: 28394

#Unit 5. Developmental Biology

the mRNA for the transcription factor VegT, which becomes apportioned to the vegetal cells during cleavage. VegT is critical in generating which of the following lineages?

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#Question id: 28395

#Unit 5. Developmental Biology

One of the genes activated by VegT protein encodes the Sox17 transcription factor , these sox17 transcription factor is critical for which functions?

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#Question id: 28396

#Unit 5. Developmental Biology

VegT is critical in generating the germ cells, two genes activated by VegT protein that encodes the transcriptional factor as well as paracrine factors that instruct the cell layers to become their fate. which of the following statements is incorrect?

a) Sox17 paracrine factor that specify cells to be endoderm

b) Nodal transcription factors that instruct the cell layers above them to become mesoderm

c) Nodal paracrine factors that instruct the cell layers above them to become mesoderm

d) Sox17 transcription factor that specify cells to be endoderm

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#Question id: 28397

#Unit 5. Developmental Biology

During the specification of amphibian axes, in the absence of any induction cell leads to become which of the following fate?

TLS Online TPP Program

#Question id: 28398

#Unit 5. Developmental Biology

Spemann referred to the dorsal lip cells and their derivatives as the organizer, these derivatives cells are___