Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

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#Question id: 3056

#Unit 2. Cellular Organization

Ten bacteria were inoculated into a rich medium. If at the end of ten hours the total number of cells is 10^4, then the number of elapsed generations and the generation time respectively is

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#Question id: 28525

#Unit 5. Developmental Biology

At which stage of plant development, Cell elongation and cellular differentiation processes occur throughout the embryonic axis?

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#Question id: 14781

#Unit 13. Methods in Biology

A pack of cards contains 4 aces, 4 queens and 4 jacks. Two cards are drawn at random. The probability that at least one of them is an ace is

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#Question id: 18922

#Unit 13. Methods in Biology

For which of the following plasmids purification of a recombinant molecule from the transformed cell can be difficult?

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#Question id: 3572

#Unit 8. Inheritance Biology

How might the ability of pea plants to self fertilize be a problem for genetic experiments?