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TLS Online TPP Program

#Question id: 13065

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Agglutination of antigens by utilising specific antibodies can only occur, if

#Unit 13. Methods in Biology
  1. The concentration of Ag or Ab is at point of equivalence
  2. At point of equivalence or Ab in excess
  3. At point of equivalence or Ag in excess
  4. All the above
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TLS Online TPP Program

#Question id: 12108

#Unit 10. Ecological Principles

Resource partitioning would be most likely to occur between
TLS Online TPP Program

#Question id: 33705

#Unit 2. Cellular Organization

Choose combination of correct statements 

           

A. The main band contains 92% of the genome
B. The main band contains 10% of the genome
C. Smaller peak represents 30% GC content of genome
D. Smaller peak represents 60% GC content of genome
TLS Online TPP Program

#Question id: 909

#Unit 1. Molecules and their Interaction Relevant to Biology

Most proteins denature at both high and low pH. At high pH, the ionization of internal tyrosines is thought to be the main destabilizing influence, whereas at low pH, the protonation of buried histidines. A titration curve for the unfolding of the enzyme ribonuclease is shown in Figure. Given the relation between the titration curves for protein unfolding and histidine protonation, which of the following conclusion can be drawn correctly?

A. The sharp transition in graph indicates a highly cooperative process; when the protein starts to unfold, it completes the process rapidly

B. The sharp transition in graph indicates a highly noncooperative process

C. Several buried histidines can ionize when the chain starts to unfold, so that when one goes, they all go together due to cooperative behaviour of protein folding

D. Buried histidine (pK of 4 in this example) becomes accessible to solvent, its pK will shift toward its normal value of 6, significantly steepening its titration curve.
TLS Online TPP Program

#Question id: 159

#Unit 1. Molecules and their Interaction Relevant to Biology

The chirality of an amino acid results from the fact that its alpha carbon:
TLS Online TPP Program

#Question id: 13121

#Unit 2. Cellular Organization

--------- act as a nuclear export factor and directs the associated mRNP to the central channel of the NPC by transiently interacting with FG- nucleoporins.