TLS Online TPP Program

#Question id: 13076


Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

#Unit 13. Methods in Biology
  1. i-ii-iii-iv-v-vi-vii
  2. vii-i-iii-ii-iv-v-vi

  3. i-iii-ii-v-iv-vi-vii
  4. i-iii-ii-iv-v-vi-vii
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#Question id: 12341

#Unit 10. Ecological Principles

The figure shows population growth trajectories over time (as indicated by the arrows) of two stored-grain beetle  species  when  grown  together  under  constant  conditions.  Three  experiments  were  performed  that  differed only in the initial densities of the two species. Which of the following is the best interpretation of the experimental results?


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#Question id: 12342

#Unit 10. Ecological Principles

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#Question id: 12343

#Unit 10. Ecological Principles

Hybrids between some related species of plants are sterile because the parent plants had different chromosome numbers. Occasionally the chromosome number of such a hybrid  plant doubles spontaneously. Which of the following best describes the descendants of those plants with the double chromosome number?

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#Question id: 12344

#Unit 10. Ecological Principles

The concept of punctuated equilibrium refers to