Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

TLS Online TPP Program

#Question id: 16114

#Unit 7. System Physiology – Animal

Which of the following hormones are thermogenic?

TLS Online TPP Program

#Question id: 16115

#Unit 7. System Physiology – Animal

Cold environmental temperatures:

TLS Online TPP Program

#Question id: 16116

#Unit 7. System Physiology – Animal

Which of the following hormones acts synergistically with sympathetic nervous system?

TLS Online TPP Program

#Question id: 16117

#Unit 7. System Physiology – Animal

Thyroid hormones are thermogenic, their actions on target tissues result in heat production by following mechanisms:

i. stimulation of Na+-K+ ATPase

ii. increased O2 consumption

iii. increased metabolic rate

iv. increase in the number and activity of mitochondria

which of the following combinations are correct regarding heat production?

TLS Online TPP Program

#Question id: 16118

#Unit 7. System Physiology – Animal

Following statements are regarding to the heat production by the activation of sympathetic nervous system.

A. Activation is stimulation of β receptors in brown fat, which increases metabolic rate and heat production.

B. Activation is stimulation of α2 receptors in brown fat, which increases metabolic rate and heat production.

C. Stimulation of α1 receptors in vascular smooth muscle of skin blood vessels, producing vasoconstriction. Vasoconstriction reduces blood flow to the surface of the skin.

D. Stimulation of α1 receptors in vascular smooth muscle of skin blood vessels, producing vasodilation. Vasodilation reduces blood flow to the surface of the skin.

Which of the following combination is correct?

TLS Online TPP Program

#Question id: 16119

#Unit 7. System Physiology – Animal

Following statements are regarding to Shivering, which is the most potent mechanism for increasing heat production in the body. 

A. Cold environmental temperatures activate centers in the posterior hypothalamus during shivering, which then activate the α and γ motoneurons innervating skeletal muscle. 

B. Shivering involves skeletal muscle contracts rhythmically, generating heat and raising body temperature.

C. Shivering involves stimulation of Na+-K+ ATPase and raising body temperature.

D. Shivering involves stimulation of β receptors in brown fat, which increases metabolic rate and heat production.

Which of the following combination is correct?