To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

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#Question id: 6998

#Unit 5. Developmental Biology

Which of the following is an adult organism that has fewer than 1,000 cells?

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#Question id: 1282

#Unit 4. Cell Communication and Cell Signaling

Basal lamina include all of the following, except:

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#Question id: 11394

#Unit 9. Diversity of Life Forms

Foods can be preserved in many ways by slowing or preventing bacterial growth. Which of these methods would not generally inhibit bacterial growth?

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#Question id: 10867

#Unit 10. Ecological Principles

Plants that inhabit stable environments tend to produce ________ seeds.

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#Question id: 19744

#Unit 3. Fundamental Processes

Which of the following statement is incorrect regarding Cdc 13