To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

TLS Online TPP Program

#Question id: 26885

#Unit 10. Ecological Principles

major anthropogenic atmospheric gases causing the greenhouse effect is

TLS Online TPP Program

#Question id: 26886

#Unit 10. Ecological Principles

Relative absorption per ppm of increase is;

CO2

Methane 

Nitrous oxide

Chlorofluorocarbon

Arrange in decreasing order

TLS Online TPP Program

#Question id: 26887

#Unit 10. Ecological Principles

Succession as proceeding to a distinct end point, each phase of succession shown in given diagram (A → D)

TLS Online TPP Program

#Question id: 26888

#Unit 10. Ecological Principles

During the rate of succession several factors would be observe at the pioneer as well as climax community;

STAGES	CHARACTER
(A) Pioneer community	(i) Biotic Potential low
(B) Climax community	(ii) Root to Shoot ratio is high
	(iii) Productivity per biomass is low
	(iv) Net primary productivity is high
	(v) P/R ratio is greater than 1
	(vi) Nutrient cycling is closed
	(vii) Regeneration ability is more
	(viii) Resistance ability is more

  Match the community stages with its given characters;

TLS Online TPP Program

#Question id: 26889

#Unit 10. Ecological Principles

Shifting cultivation is a form of agricultural practice in the hills of Northeast India, where a forest patch is cleared and cultivated for a few years, after which it is left fallow and allowed to regenerate. Succession occurs on such previously cultivated plots. Over time, the plant community should move towards which region in the graph?

TLS Online TPP Program

#Question id: 26890

#Unit 10. Ecological Principles

The MacArthur-Wilson equilibrium model of island biogeography predicts the number of species found on islands. The figure below depicts two volcanic islands located off a section of mainland coast.

 The MacArthur-Wilson model predicts that the relative numbers of bird species on the mainland and two islands would be